Journal: bioRxiv
Article Title: An Innate Immune Receptor Toll-1 converts chronic light stress into glial-phagocytosis
doi: 10.1101/2025.09.03.673940
Figure Lengend Snippet: (A–G’) Co-localization analysis of Toll and subcellular compartment markers in Drosophila S2 cells. Toll-1::Venus was expressed by co-transfection of pActin-GAL4 and p20×UAS-Toll-1::Venus plasmids, followed by immunostaining to assess co-localization. (A and A’) Toll-1::Venus anti-Rab5 labeling. (B and B’) Toll-1::Venus anti-Rab7 labeling. (C and C’) Toll-1::Venus anti-Rab8 labeling. (D and D’) Toll-1::Venus anti-Atg8 labeling. (E and E’) Toll-1::Venus anti-Kdel labeling. (F and F’) Toll-1::Venus anti-Lamp1 labeling. (G and G’) Toll-1::Venus anti-Fmr1 labeling. (H) Quantification of co-localization using Pearson’s correlation coefficients between Toll-1::Venus and various organelle markers: stress granules (Fmr1), lysosomes (Lamp1), endoplasmic reticulum (Kdel), autophagosomes (Atg8), early endosomes (Rab5), secretory vesicles (Rab8), and late endosomes (Rab7).
Article Snippet: The following antibodies were used for immunohistochemistry: mAb24B10 (1:25, DSHB), rat antibody to HA (3F10, 1:50, Roche), mouse antibody to Fmr1 (5A11, 1:50, DSHB), rabbit antibody to Lamp1 (1:200, Abcam), mouse antibody to KDEL (10C3, 1:50, Novus), rabbit antibody to Atg8 (1:200, Abcam), rabbit antibody to Rab5 (1:50, Abcam), mouse antibody to Rab8 (1:400, BD Biosciences), and mouse antibody to Rab7 (1:100, DSHB).
Techniques: Cotransfection, Immunostaining, Labeling